Protocol and procedure for creating adult stem cells

1) We use peripheral blood cells, bone marrow biopsy and fat tissue

To collect and harvest the stem cells we use different techniques. First, we stimulate the bone marrow to produce more stem cells, this way when we draw the peripheral blood, we get high concentration of stem cells. We then draw 20cc of blood.

2) The second technique we use is the bone marrow biopsy where se use a hassida needle to get the bone marrow from the iliac crest. We also look for perone bone, the leg bone and sternum. These three areas have enough high concentration of stem cells which we then draw approximately 100-500 cc or one liter of blood.

3) When we extract stem cells from fat issue, we use the abdomen as the location of the incision, (around the belly button) and get the subcutaneous fat. We remove approx. 5 gram of fat issue and with this sample we separate the fat and harvest the rest of the tissue in the laboratory. We use specific enzymes to break the collagen of the far; we remove the stroma leaving mesenquima cells only which are the targeted stem cells. We create special cultures that we place in an incubator and then we select what kind of organ to regenerate. We use specific cytokines for the organ that we want.

The blood from the bone marrow and peripheral blood is separated with ficoll isolating as we harvest stem cells. We add growth factors, nutrition and cytokines to this culture before we inject back into the patient.

Administering adult stems

In order to grow stem cells in the lab specifically for each patient we collect the bone marrow in one pint of blood. We inject growth and cytokines for differentiation of the stem cells. At the same time, we get plasma from patient and we get growth factors and reapply it to the patient through the IV

This technique is unique and when the doctors don’t have the laboratories to select grow and the stem cells separate, they instead apply the stem cells immediately to patients. The stem cells then begin the signal process, the main signal is finding an inflammatory reaction. For example, if a patient has hepatitis and inflammatory of the liver, the stem cells will fix or repair the liver. The body normally has about 10% stem cells working to fix any inflammation happening in the body. This is enough cell to repair the liver or any organ, so we boost the stem cells to 90% and this will repair the organ and diminish the inflammatory process.

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